Advances in Micropipette Aspiration: Applications in Cell Biomechanics, Models, and Extended Studies
نویسندگان
چکیده
منابع مشابه
Characterizing cell adhesion by using micropipette aspiration.
We have developed a technique to directly quantify cell-substrate adhesion force using micropipette aspiration. The micropipette is positioned perpendicular to the surface of an adherent cell and a constant-rate aspiration pressure is applied. Since the micropipette diameter and the aspiration pressure are our control parameters, we have direct knowledge of the aspiration force, whereas the cel...
متن کاملMicropipette aspiration of living cells.
The mechanical behavior of living cells is studied with micropipette suction in which the surface of a cell is aspirated into a small glass tube while tracking the leading edge of its surface. Such edges can be tracked in a light microscope to an accuracy of +/-25 nm and suction pressures as small as 0.1-0.2 pN/microm2 can be imposed on the cell. Both soft cells, such as neutrophils and red cel...
متن کاملPerformance studies in micropipette calibration
The measurement of small amounts of liquids is very important in fields like, health, chemistry, microbiology and genetics. The most common used instruments for delivery of liquids, in the order of the microliter, are micropipettes or piston pipettes, of fixed or variable volume, with positive or air displacement operation. The method used in the calibration of micropipettes is the gravimetric ...
متن کاملMechanics and deformation of the nucleus in micropipette aspiration experiment.
Robust biomechanical models are essential for the study of nuclear mechanics and deformation and can help shed light on the underlying mechanisms of stress transition in nuclear elements. Here, we develop a computational model for an isolated nucleus undergoing micropipette aspiration. Our model includes distinct components representing the nucleoplasm and nuclear envelope. The nuclear envelope...
متن کاملEstimating Young's modulus of zona pellucida by micropipette aspiration in combination with theoretical models of ovum.
The zona pellucida (ZP) is the spherical layer that surrounds the mammalian oocyte. The physical hardness of this layer plays a crucial role in fertilization and is largely unknown because of the lack of appropriate measuring and modelling methods. The aim of this study is to measure the biomechanical properties of the ZP of human/mouse ovum and to test the hypothesis that Young's modulus of th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2019
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.01.004